Ca-measurement in glandular tissue

Posted by Cselenyak Attila on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Ca-measurement-in-glandular-tissue-tp5035189.html

Dear List members,

we would like measure Ca(2+) in glandular tissue with Fluo3 using
confocal mircoscopy. We tried to load the tissue pieces with different
concentrations of the dye for 1,5 hours in 37C, washing after that
with PBS, and the tissue was put in a perfusion system. But we could
not see any changes in the fluorescence intensity when giving either
ATP or A23187 ionophore to the perfusion system.

Do you have any experience what would be an optimal loading protocol
or Fluo3 is suitable for this kind of measurements?

Thanks is advence!

Best regards,

Attila

--
Attila Cselenyak
PhD student

Semmelweis University
Basic Medical Science Center
Institute Of Human Physiology And Clinical Experimental Research
H-1094, Budapest
Tűzoltó utca 37-47.
Phone: [phone] ext.60342
Mobile: [phone]