We did collect calcium signal in the tissue using calcium green,
but we injected the dye. We are getting pretty good signals.
Hope this helps.
Ammasi Periasamy, Ph.D.
Director, Keck Center for Cellular Imaging (KCCI)
Professor of Biology and Biomedical Engineering
Biology, Gilmer Hall (064), 485 McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:[hidden email]
http://www.kcci.virginia.edu
************************
10th Annual Workshop on FRET Microscopy, March 8-12, 2011
http://www.kcci.virginia.edu/workshop/workshop2011/index.php
*************************
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of [hidden email]
Sent: Tuesday, May 11, 2010 8:42 AM
To: [hidden email]
Subject: RE Ca-measurement in glandular tissue
Hi Attila,
Do you see any
fluorescence? Does fluo3 etting inside the tissue? Maybe adding some
pluronic acid can help to have a better signal!
Have a nice
day,
Louisssssssss
|
Cselenyak
Attila <[hidden email]>@LISTS.UMN.EDU 2010-05-11
05:41
|
|
Dear List members,
we would like measure Ca(2+) in glandular tissue with Fluo3 using
confocal mircoscopy. We tried to load the tissue pieces with different
concentrations of the dye for 1,5 hours in 37C, washing after that
with PBS, and the tissue was put in a perfusion system. But we could
not see any changes in the fluorescence intensity when giving either
ATP or A23187 ionophore to the perfusion system.
Do you have any experience what would be an optimal loading protocol
or Fluo3 is suitable for this kind of measurements?
Thanks is advence!
Best regards,
Attila
--
Attila Cselenyak
PhD student
Semmelweis University
Basic Medical Science Center
Institute Of Human Physiology And Clinical Experimental Research
H-1094, Budapest
Tűzoltó utca 37-47.
Phone: +36-1-4591500 ext.60342
Mobile: +36-70-3874931
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