http://confocal-microscopy-list.275.s1.nabble.com/Embryos-imaging-tp5085651p5086719.html
Try mounting in low melting point agarose. To dissolve in your buffer
you need to heat to about 60 deg C. But then you can cool it down to
37deg C and it will stay molten until cooled to 35 deg C. Once
solidified you can warm to 37deg again. It is perfect for imaging
Stephen H. Cody
> Dear Alice,
>
> Thank you for your reply.
> Unfortunately, we have only LSM confocal. Could you clarify about the
> Teflon film please?
>
> With regards,
> Vladimir
>
>
> On Fri, May 21, 2010 at 2:29 PM, Alice Rodriguez Diaz
> <
[hidden email]> wrote:
>> Dear Vladimir,
>>
>> Fast imaging with a spinning disk confocal. And Teflon film to
>> allow air and
>> movement.. ?
>>
>> Alice
>>
>> -----Original Message-----
>> From: Confocal Microscopy List
>> [mailto:
[hidden email]] On
>> Behalf Of Vladimir Gukassyan
>> Sent: Friday, May 21, 2010 1:14 PM
>> To:
[hidden email]
>> Subject: Embryos imaging
>>
>> Dear List members,
>>
>> We're trying to do blastocysts confocal imaging but face a problem of
>> active movement. They are pretty reluctant to adhesions, hence the
>> difficulty with immobilization. Did you face the same problem, and if
>> yes - what would be the suggestion to try to overcome it?
>>
>> Thank you in advance,
>> Vladimir
>>