I use dental wax. Since we are an EM lab as well as optical we
always have some on a hotplate. It sets pretty much instantly as it cools and does
not seem to harm even the most delicate tissue.
Guy
Please help fight breast cancer by sponsoring my
run in the Sydney Half Marathon on May 16th.
http://www.everydayhero.com.au/guy_cox
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Shalin Mehta
Sent: Sunday, 23 May 2010 1:23 PM
To: [hidden email]
Subject: Sealing the specimen mounted in cargille oils
Hello listers,
I am using Cargille immersion oil for mounting some test targets. I am looking
for a good sealant that does not react with cargille immersion oils. Nail
polish tends to mix with oils as it dries slowly. I am trying to avoid
VALAP because it spreads on the coverslip if scratched accidentally. Is there
something that will dry fast and not mix too much with the immersion oils?
Thanks for suggestions
Shalin
blog: shalin.wordpress.com
(Current:) Visiting Researcher at Oldenbourg Lab, Marine Biological Laboratory.
Lillie 110, 7 MBL Street, Woods Hole, MA 02543, USA.
(Permanent:)
Bioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm
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