Re: Embryos imaging

Posted by Barry O'Brien on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Embryos-imaging-tp5085651p5092232.html

Infiltration with glycerol over a couple of days helps.  Temperature
equilibration is also an area to pay attention to.

If anyone has a reliable method of stabilizing the embryo after
orienting it while on the microscope stage then we would also like to
hear about it.

Thanks,

Barry

At 06:13 a.m. 22/05/2010, you wrote:
>Dear List members,
>
>We're trying to do blastocysts confocal imaging but face a problem of
>active movement. They are pretty reluctant to adhesions, hence the
>difficulty with immobilization. Did you face the same problem, and if
>yes - what would be the suggestion to try to overcome it?
>
>Thank you in advance,
>Vladimir

Dr Barry O'Brien
Dept of Biological Sciences,
University of Waikato
Private Bag 3105
HAMILTON
New Zealand

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