Re: Sealing the specimen mounted in cargille oils

Posted by Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Sealing-the-specimen-mounted-in-cargille-oils-tp5089866p5099581.html

Silicone grease may work as a last resort, but using it around lenses always gives me the willies.  

Regards,Glen

On May 25, 2010, at 10:23 AM, Dave McDonald wrote:

> In the past I've used rubber cement with success for other samples.  Never tried using it with immersion oil as a mounting medium though....
>
> Dave
>
> Dave McDonald
> Scientific Imaging Lab
> Fred Hutchinson Cancer Research Center
> 1100 Fairview Avenue North, DE-512
> Seattle, WA 98109
> 206-667-4205
> http://www.fhcrc.org
>
> At 08:22 PM 5/22/2010, you wrote:
>> Hello listers,
>> I am using Cargille immersion oil for mounting some test targets. I am looking for  a good sealant that does not react with cargille immersion oils. Nail polish  tends to mix with oils as it dries slowly. I am trying to avoid VALAP because it spreads on the coverslip if scratched accidentally. Is there something that will dry fast and not mix too much with the immersion oils?
>>
>> Thanks for suggestions
>>
>> Shalin
>> blog: shalin.wordpress.com
>>
>> (Current:) Visiting Researcher at Oldenbourg Lab, Marine Biological Laboratory.
>> Lillie 110, 7 MBL Street, Woods Hole, MA 02543, USA.
>>
>> (Permanent:)
>> Bioimaging Lab, Block-E3A, #7-10                
>> Div of Bioengineering, NUS Singapore 117574  
>> website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm

Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]