Posted by
Eric Scarfone on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-CONFOCALMICROSCOPY-Digest-25-May-2010-to-26-May-2010-2010-16-tp5107012p5108111.html
I "love" this thread!
Eric
Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet
Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden
Work: +46 (0)8-517 79343,
Cell: +46 (0)70 888 2352
Fax: +46 (0)8-301876
email: [hidden email]
http://www.ki.se/cfh/
----- Original Message -----
From: Christophe Leterrier <[hidden email]>
Date: Thursday, May 27, 2010 11:58 am
Subject: Re: CONFOCALMICROSCOPY Digest - 25 May 2010 to 26 May 2010 (#2010-16)
To: [hidden email]
> Lots of love for you all !
>
> Christophe
>
> On Thu, May 27, 2010 at 11:38, Peter Pitrone <
[hidden email]>
> wrote:> Jason,
> >
> > I love you too...
> >
> > Smooches,
> >
> > On May 27, 2010, at 07:10 AM,
[hidden email] wrote:
> >
> >> If you are getting this in the morning, good morning! I love you!
> >> Jason Brenner
> >> Microscope Sales Representative
> >> Olympus America, Inc.
> >> 107 Kay Street
> >> Ithaca, NY 14850
> >> Mobile: 607-592-1200
> >>
[hidden email] > >> http://www.olympusamerica.com/seg_section/seg_home.asp
> >>
> >>
> >> ----- Original Message -----
> >> From: CONFOCALMICROSCOPY automatic digest system
> [
[hidden email]]>> Sent: 05/27/2010 12:02 AM EST
> >> To:
[hidden email] > >> Subject: CONFOCALMICROSCOPY Digest - 25 May 2010 to 26 May 2010
> (#2010-16)
> >>
> >>
> >>
> >> There are 9 messages totalling 585 lines in this issue.
> >>
> >> Topics of the day:
> >>
> >> 1. colocalization and focus *commercial response*
> >> 2. Primary dochroics in A1r
> >> 3. Resonant scanner from A1R vs SP5 ...
> >> 4. AUTO: Dupal, Mark is out of the office. (returning 28/05/2010)
> >> 5. Poor man's confocal (5)
> >>
> >> ----------------------------------------------------------------
> ------
> >>
> >> Date: Wed, 26 May 2010 09:27:29 +0200
> >> From: Daniel James White <
[hidden email]>
> >> Subject: Re: colocalization and focus *commercial response*
> >>
> >> Hi Kevin and Carl,
> >>
> >>
> >> Begin forwarded message:
> >>
> >>> =20
> >>> Hi Carl,
> >>> =20
> >>> According to Bitplane's records, there is a permanent license
> for =
> >> Imaris=20
> >>> 5.5 registered to you.
> >>> Although you can't upgrade it or get support without renewing=20
> >>> maintenance, I'm pretty sure that version has the necessary
> components=20=>>
> >>> to apply channel shift corrections.
> >>> The simplest approach would be via the Image Processing menu
> if Imaris=20=
> >>
> >>> ("Channel Shift") -- but the shift units are in pixels/voxels,
> so you=20=
> >>
> >>> would have to resample the dataset first to achieve sub-voxel
> shift.>>
> >> if you resample the data to smaller pixels you need to be very
> sure how =
> >> that is done,=20
> >> so that you dont destroy the intensity data in the image.=20
> >> Intensities need to be kept nice, since that how the coloc
> methods asses =
> >> goodness of signal overlap.=20
> >>
> >> Perhaps Kevin can explain the maths behind how imaris resamples
> an image =
> >> to smaller pixels.=20
> >> I dont see a good explanation in the docs... maybe i missed it.=20
> >>
> >> The only safe resample method is a whole pixel binning...=20
> >> but that making the pixels bigger.=20
> >> To make them smaller you need to interpolate somehow...
> >> and the way you do that is critical for not destroying the
> image =
> >> intensity data.=20
> >>
> >> Kevin?
> >>
> >>> =20
> >>> If you had the ImarisTrack module (unfortunately looks like
> you =
> >> don't),=20
> >>> there is also a "Drift Correction" function that could be
> applied with=20=
> >>
> >>> sub-voxel precision automatically (first would need to swap
> the time &=20=
> >>
> >>> channel axes, then correct drift, then swap them back again).=20
> >>
> >> Again an explanation of how that really does the maths would be
> really =
> >> great,=20
> >> especially since one must have no black boxes.=20
> >> or else one can not publish honestly.=20
> >>
> >> Where do i find a mathematical explanation of how this sub
> pixel image =
> >> shifting is done,=20
> >> and how can i be sure image intensities are not destroyed do to
> a bad =
> >> interpolation method?
> >> Is it in the docs?
> >>
> >> cheers
> >>
> >> Dan
> >>
> >>
> >>
> >>> =20
> >>> Best regards,
> >>> -Kevin
> >>> =20
> >>> Kevin Frischmann
> >>> Head of Technical Support, US & Canada
> >>> Bitplane, Inc.
> >>> tel: +1 888-332-4879, ext. 11
> >>> fax: 866-691-9112
> >>>
[hidden email] > >>
> >> Dr. Daniel James White BSc. (Hons.) PhD
> >> Senior Microscopist / Image Visualisation, Processing and Analysis
> >> Light Microscopy and Image Processing Facilities=20
> >> Max Planck Institute of Molecular Cell Biology and Genetics
> >> Pfotenhauerstrasse 108
> >> 01307 DRESDEN
> >> Germany
> >>
> >> +49 (0)15114966933 (German Mobile)
> >> +49 (0)351 210 2627 (Work phone at MPI-CBG)
> >> +49 (0)351 210 1078 (Fax MPI-CBG LMF)
> >>
> >> http://www.bioimagexd.net BioImageXD
> >> http://pacific.mpi-cbg.de Fiji - is just ImageJ =
> >> (Batteries Included)
> >> http://www.chalkie.org.uk Dan's Homepages
> >> https://ifn.mpi-cbg.de Dresden Imaging
> Facility Network
> >> dan (at) chalkie.org.uk
> >> ( white (at) mpi-cbg.de )
> >>
> >> ------------------------------
> >>
> >> Date: Wed, 26 May 2010 11:23:56 +0200
> >> From: Juan Luis Ribas <
[hidden email]>
> >> Subject: Primary dochroics in A1r
> >>
> >> Dear list,
> >> Does anyone have access to the transmision curves from the low-
> angle>> incidence dichroic mirrors installed in the Nikon A1r?
> >>
> >> Best regards
> >>
> >> Juan Luis
> >>
> >> --
> >> Juan Luis Ribas
> >> Servicio de Microscopía
> >> Centro de Investigación, Tecnología e Innovación
> >> Universidad de Sevilla
> >> Av. Reina Mercedes 4b
> >> 41012 Sevilla
> >>
> >> Tfno: 954559983
> >>
> >> ------------------------------
> >>
> >> Date: Wed, 26 May 2010 20:35:07 +0530
> >> From: Roshma Azeem <
[hidden email]>
> >> Subject: Re: Resonant scanner from A1R vs SP5 ...
> >>
> >> --0016369207427c784004878099f8
> >> Content-Type: text/plain; charset=ISO-8859-1
> >>
> >> Hi Mac,
> >>
> >> Fast acquisition time is required to analyze rapid biological
> processes in a
> >> cell. Resonant scanners are about 10 times faster compared to
> the speed of
> >> conventional scanners that are able to acquire fast frame
> recording and
> >> provide real time live images. Due to the faster frame rate,
> they are useful
> >> in resolving the complicated dynamic changes in living cells.
> >>
> >> Resonant scanners have some disadvantages like higher readout
> noise. In
> >> addition, their duty cycle is short as resonant scanners
> accelerate fast
> >> that may affect the scanning mirror .
> >>
> >> Further technical information can be seen at the following sites:
> >>
> >>
> http://www.microscopyu.com/tutorials/flash/resonantscanning/confocalresonantscanning/index.html>>
> >> http://www.microscopyu.com/articles/confocal/resonantscanning.html
> >>
> >>
> >> Nikon's A1R has a hybrid scanner and the SP5 has tandem scanner.
> >>
> >>
> >> Roshma.
> >>
> >> --0016369207427c784004878099f8
> >> Content-Type: text/html; charset=ISO-8859-1
> >> Content-Transfer-Encoding: quoted-printable
> >>
> >> <br>Hi Mac, <br><br>Fast acquisition time is required to
> analyze rapid biol=
> >> ogical=20
> >> processes in a cell. Resonant scanners are about 10 times
> faster compared t=
> >> o the speed of conventional scanners that are able to acquire
> fast frame re=
> >> cording and provide real time live images. Due to the faster
> frame rate, th=
> >> ey are useful in resolving the complicated dynamic changes in=20
> >> living cells.<br><br>Resonant scanners have some disadvantages
> like higher =
> >> readout noise. In addition, their duty cycle is short as
> resonant scanners =
> >> accelerate fast that may affect the scanning mirror
> .<br><br>Further techni=
> >> cal information can be seen at the following sites: <br>
> >> <br>http://www.microscopyu.com/tutorials/flash/resonantscanning/=
> >>
> confocalresonantscanning/index.html">http://www.microscopyu.com/tutorials/f=>> lash/resonantscanning/confocalresonantscanning/index.html<br><br>>> f=3D"http://www.microscopyu.com/articles/confocal/resonantscanning.html">ht=
> >>
> tp://www.microscopyu.com/articles/confocal/resonantscanning.html<br>>> <br><br>Nikon's A1R has a hybrid scanner and the SP5 has tandem scanner=
> >> . <br><br><br>Roshma.<br>
> >>
> >> --0016369207427c784004878099f8--
> >>
> >> ------------------------------
> >>
> >> Date: Thu, 27 May 2010 04:00:28 +1000
> >> From: Mark Dupal <
[hidden email]>
> >> Subject: AUTO: Dupal, Mark is out of the office. (returning
> 28/05/2010)>>
> >> I am out of the office until 28/05/2010.
> >>
> >> I will have limited email access and will respond to your
> message when I
> >> return.
> >>
> >>
> >> Note: This is an automated response to your message "Re:
> Resonant scanner
> >> from A1R vs SP5 ..." sent on 5/27/2010 1:05:07 AM.
> >>
> >> This is the only notification you will receive while this
> person is away.
> >>
> >> P Please consider the environment before printing this email -
> 3 sheets o=
> >> f A4 paper =3D 1 litre of water This message is intended only
> for the addre=
> >> ssee. If you are not the intended recipient you are notified
> that disclos=
> >> ing, copying, distributing or taking any action in reliance of
> the contents=
> >> of this information is strictly prohibited. =
> >>
> >> ------------------------------
> >>
> >> Date: Wed, 26 May 2010 11:32:12 -0700
> >> From: Bob Nienhuis <
[hidden email]>
> >> Subject: Poor man's confocal
> >>
> >> --0016362837d817d8ce0487837ecd
> >> Content-Type: text/plain; charset=ISO-8859-1
> >>
> >> Anybody have any experience with the Zeiss Apotome structured
> illumination>> system?
> >>
> >> I am writing a proposal for a new microscope system and the
> vendor mentioned
> >> this as a way
> >> to reduce background in fluorescence microscopy.
> >>
> >> Apparently, it superimposes a moving grid on the image, and
> this somehow
> >> allows background
> >> noise reduction.
> >>
> >> We have used deconvolution to do this, but find that it takes a
> long time to
> >> do.
> >>
> >> I think it would add about $20k to the cost of the scope.
> >>
> >> Opinions? Worthwhile? Any experience with it?
> >>
> >> Bob Nienhuis
> >> Neurobiology Research M/S151A3
> >> UCLA / VA Medical Center
> >> North Hills, CA
> >>
[hidden email] > >>
> >> --0016362837d817d8ce0487837ecd
> >> Content-Type: text/html; charset=ISO-8859-1
> >> Content-Transfer-Encoding: quoted-printable
> >>
> >> <div>Anybody have any experience with the Zeiss Apotome
> structured illumina=
> >> tion system?</div>
> >> <div>=A0</div>
> >> <div>I am writing a proposal for a new microscope system and
> the vendor men=
> >> tioned this as a way</div>
> >> <div>to reduce background in fluorescence microscopy.</div>
> >> <div>=A0</div>
> >> <div>Apparently, it superimposes a moving grid on the image,
> and this someh=
> >> ow allows background</div>
> >> <div>noise reduction. </div>
> >> <div>=A0</div>
> >> <div>We have used deconvolution to do this, but find that it
> takes a long t=
> >> ime to do.</div>
> >> <div>=A0</div>
> >> <div>I think it would add about $20k to the cost of the
> scope.</div>>> <div>=A0</div>
> >> <div>Opinions? Worthwhile? Any experience with it?</div>
> >> <div>=A0</div>
> >> <div>Bob Nienhuis</div>
> >> <div>Neurobiology Research M/S151A3</div>
> >> <div>UCLA / VA Medical Center</div>
> >> <div>North Hills, CA </div>
> >> <div>mailto:
[hidden email]">
[hidden email]</=
> >> div>
> >>
> >> --0016362837d817d8ce0487837ecd--
> >>
> >> ------------------------------
> >>
> >> Date: Wed, 26 May 2010 14:44:53 -0400
> >> From: Joel Sheffield <
[hidden email]>
> >> Subject: Re: Poor man's confocal
> >>
> >> There has been a great deal of discusion of structured illumination
> >> recently. You might take a look at NATUREMETHODS VOL.6NO.5:339
> >> for one example. In addition to the Apotome modification,
> there is
> >> also an add-on for other microscopes by Optigrid.
> >> (http://www.qioptiqlinos.com/Products/StructuredLightSystem/)
> >>
> >> I haven't used either system, but I am also interested. Let me
> know>> what you find out.
> >>
> >> Joel
> >>
> >> -------------- Original message ---------------
> >> Anybody have any experience with the Zeiss Apotome structured
> >> illumination system?
> >>
> >> I am writing a proposal for a new microscope system and the vendor
> >> mentioned this as a way
> >> to reduce background in fluorescence microscopy.
> >>
> >> Apparently, it superimposes a moving grid on the image, and this
> >> somehow allows background
> >> noise reduction.
> >>
> >> We have used deconvolution to do this, but find that it takes a
> long>> time to do.
> >>
> >> I think it would add about $20k to the cost of the scope.
> >>
> >> Opinions? Worthwhile? Any experience with it?
> >>
> >> Bob Nienhuis
> >> Neurobiology Research M/S151A3
> >> UCLA / VA Medical Center
> >> North Hills, CA
> >>
[hidden email] > >> --
> >> Joel B. Sheffield, Ph.D.
> >> Biology Department, Temple University
> >> 1900 North 12th Street
> >> Philadelphia, PA 19122
> >>
[hidden email] > >> (215) 204 8839, fax (215) 204 0486
> >> http://astro.temple.edu/~jbs
> >>
> >> ------------------------------
> >>
> >> Date: Wed, 26 May 2010 15:52:27 -0400
> >> From: Dale Callaham <
[hidden email]>
> >> Subject: Re: Poor man's confocal
> >>
> >> I have just finished training a user who came to us because the
> Apotome>> system did not give anything like the results of the
> LSM510. I certainly
> >> don't mean to suggest the Apotome system doesn't work, but you
> should>> get a demo on your samples to see how it works for you.
> >>
> >> Dale
> >>
> >> Joel Sheffield wrote:
> >>> There has been a great deal of discusion of structured
> illumination>>> recently. You might take a look at NATUREMETHODS
> VOL.6NO.5:339>>> for one example. In addition to the Apotome
> modification, there is
> >>> also an add-on for other microscopes by Optigrid.
> >>> (http://www.qioptiqlinos.com/Products/StructuredLightSystem/)
> >>>
> >>> I haven't used either system, but I am also interested. Let
> me know
> >>> what you find out.
> >>>
> >>> Joel
> >>>
> >>> -------------- Original message ---------------
> >>> Anybody have any experience with the Zeiss Apotome structured
> >>> illumination system?
> >>>
> >>> I am writing a proposal for a new microscope system and the vendor
> >>> mentioned this as a way
> >>> to reduce background in fluorescence microscopy.
> >>>
> >>> Apparently, it superimposes a moving grid on the image, and this
> >>> somehow allows background
> >>> noise reduction.
> >>>
> >>> We have used deconvolution to do this, but find that it takes
> a long
> >>> time to do.
> >>>
> >>> I think it would add about $20k to the cost of the scope.
> >>>
> >>> Opinions? Worthwhile? Any experience with it?
> >>>
> >>> Bob Nienhuis
> >>> Neurobiology Research M/S151A3
> >>> UCLA / VA Medical Center
> >>> North Hills, CA
> >>>
[hidden email]
> >>> --
> >>> Joel B. Sheffield, Ph.D.
> >>> Biology Department, Temple University
> >>> 1900 North 12th Street
> >>> Philadelphia, PA 19122
> >>>
[hidden email] > >>> (215) 204 8839, fax (215) 204 0486
> >>> http://astro.temple.edu/~jbs
> >>
> >> ------------------------------
> >>
> >> Date: Wed, 26 May 2010 15:00:05 -0500
> >> From: "Vergara, Leoncio A." <
[hidden email]>
> >> Subject: Re: Poor man's confocal
> >>
> >> I been told the images of the apotome are not quantitative...
> If that is t=
> >> rue, that seems to me a big limitation of using the apotome for
> any serious=
> >> fluorescence microscopy application.=20
> >>
> >> Leoncio=20
> >>
> >> -----Original Message-----
> >> From: Confocal Microscopy List
> [mailto:
[hidden email]] On=
> >> Behalf Of Dale Callaham
> >> Sent: Wednesday, May 26, 2010 2:52 PM
> >> To:
[hidden email] > >> Subject: Re: Poor man's confocal
> >>
> >> I have just finished training a user who came to us because the
> Apotome sys=
> >> tem did not give anything like the results of the LSM510. I
> certainly don't=
> >> mean to suggest the Apotome system doesn't work, but you should
> get a demo=
> >> on your samples to see how it works for you.
> >>
> >> Dale
> >>
> >> Joel Sheffield wrote:
> >>> There has been a great deal of discusion of structured
> illumination=20>>> recently. You might take a look at
> NATUREMETHODS VOL.6NO.5:339 for=20
> >>> one example. In addition to the Apotome modification, there
> is also=20
> >>> an add-on for other microscopes by Optigrid.
> >>> (http://www.qioptiqlinos.com/Products/StructuredLightSystem/)
> >>>
> >>> I haven't used either system, but I am also interested. Let
> me know=20
> >>> what you find out.
> >>>
> >>> Joel
> >>>
> >>> -------------- Original message --------------- Anybody have
> any=20>>> experience with the Zeiss Apotome structured
> illumination system?
> >>>
> >>> I am writing a proposal for a new microscope system and the
> vendor=20>>> mentioned this as a way to reduce background in
> fluorescence=20>>> microscopy.
> >>>
> >>> Apparently, it superimposes a moving grid on the image, and
> this=20>>> somehow allows background noise reduction.
> >>>
> >>> We have used deconvolution to do this, but find that it takes
> a long=20
> >>> time to do.
> >>>
> >>> I think it would add about $20k to the cost of the scope.
> >>>
> >>> Opinions? Worthwhile? Any experience with it?
> >>>
> >>> Bob Nienhuis
> >>> Neurobiology Research M/S151A3
> >>> UCLA / VA Medical Center
> >>> North Hills, CA
> >>>
[hidden email] > >>> --
> >>> Joel B. Sheffield, Ph.D.
> >>> Biology Department, Temple University
> >>> 1900 North 12th Street
> >>> Philadelphia, PA 19122
> >>>
[hidden email] > >>> (215) 204 8839, fax (215) 204 0486
> >>> http://astro.temple.edu/~jbs
> >>
> >> ------------------------------
> >>
> >> Date: Thu, 27 May 2010 09:35:43 +0530
> >> From: Roshma Azeem <
[hidden email]>
> >> Subject: Re: Poor man's confocal
> >>
> >> --001636e0a6c4223f7c04878b813a
> >> Content-Type: text/plain; charset=ISO-8859-1
> >>
> >> Hi Bob,
> >>
> >> The performance of a structured illumination system may be
> close to the
> >> capabilities of a confocal microscope but this cannot be an
> alternative for
> >> CLSM. The performance difference is considerably high and
> Apotome has no
> >> flexibility like confocal. This can be used as entry level optical
> >> sectioning equipment.
> >>
> >> In my opinion, this can be used as a pre-scanning system before
> we do
> >> specimen analysis with CLSM. We can filter the number of
> specimen to be
> >> analyzed with CLSM.
> >>
> >> If I am not mistaken, Apotome cannot be fitted with any other
> microscope as
> >> it is designed for Zeiss systems. However, OptiGrid can be
> fitted with any
> >> existing microscopes. In addition, the buyer can select the
> CCD/EMCCD of
> >> their choice.
> >>
> >> Why not consider going for an entry level CLSM like Nikon C1
> plus or Olympus
> >> FV10i that may cost less than or almost the same cost of
> Apotome +
> >> Microscope, but you get the real uncompromising result of confocal
> >> microscopy?
> >>
> >> Roshma.
> >>
> >>
> >>
> >> On Thu, May 27, 2010 at 12:02 AM, Bob Nienhuis
> <
[hidden email]>wrote:>>
> >>> Anybody have any experience with the Zeiss Apotome structured
> illumination>>> system?
> >>>
> >>> I am writing a proposal for a new microscope system and the vendor
> >>> mentioned this as a way
> >>> to reduce background in fluorescence microscopy.
> >>>
> >>> Apparently, it superimposes a moving grid on the image, and
> this somehow
> >>> allows background
> >>> noise reduction.
> >>>
> >>> We have used deconvolution to do this, but find that it takes
> a long time
> >>> to do.
> >>>
> >>> I think it would add about $20k to the cost of the scope.
> >>>
> >>> Opinions? Worthwhile? Any experience with it?
> >>>
> >>> Bob Nienhuis
> >>> Neurobiology Research M/S151A3
> >>> UCLA / VA Medical Center
> >>> North Hills, CA
> >>>
[hidden email] > >>>
> >>
> >> --001636e0a6c4223f7c04878b813a
> >> Content-Type: text/html; charset=ISO-8859-1
> >> Content-Transfer-Encoding: quoted-printable
> >>
> >> <br>Hi Bob,<br><br>The performance of a structured illumination
> system may =
> >> be close to the capabilities of a confocal microscope but this
> cannot be an=
> >> alternative for CLSM. The performance difference is
> considerably high and =
> >> Apotome has no flexibility like confocal. This can be used as
> entry level o=
> >> ptical sectioning equipment. <br>
> >> <br>In my opinion, this can be used as a pre-scanning system
> before we do s=
> >> pecimen analysis with CLSM. We can filter the number of
> specimen to be anal=
> >> yzed with CLSM. <br><br>If I am not mistaken, Apotome cannot be
> fitted with=
> >> any other microscope as it is designed for Zeiss systems.
> However, OptiGri=
> >> d can be fitted with any existing microscopes. In addition, the
> buyer can s=
> >> elect the CCD/EMCCD of their choice.<br>
> >> <br>Why not consider going for an entry level CLSM like Nikon
> C1 plus or Ol=
> >> ympus FV10i that may cost less than or almost the same cost of
> Apotome + Mi=
> >> croscope, but you get the real uncompromising result of
> confocal microscopy=
> >> ?<br>
> >> <br>Roshma.<br><br><br><br><div class=3D"gmail_quote">On Thu,
> May 27, 2010 =
> >> at 12:02 AM, Bob Nienhuis <span dir=3D"ltr"><mailto:bob.nienh=
> >>
[hidden email]">
[hidden email]></span>
> wrote:<br><blockquote =
> >> class=3D"gmail_quote" style=3D"margin: 0pt 0pt 0pt 0.8ex;
> border-left: 1px =
> >> solid rgb(204, 204, 204); padding-left: 1ex;">
> >> <div>Anybody have any experience with the Zeiss Apotome
> structured illumina=
> >> tion system?</div>
> >> <div>=A0</div>
> >> <div>I am writing a proposal for a new microscope system and
> the vendor men=
> >> tioned this as a way</div>
> >> <div>to reduce background in fluorescence microscopy.</div>
> >> <div>=A0</div>
> >> <div>Apparently, it superimposes a moving grid on the image,
> and this someh=
> >> ow allows background</div>
> >> <div>noise reduction. </div>
> >> <div>=A0</div>
> >> <div>We have used deconvolution to do this, but find that it
> takes a long t=
> >> ime to do.</div>
> >> <div>=A0</div>
> >> <div>I think it would add about $20k to the cost of the
> scope.</div>>> <div>=A0</div>
> >> <div>Opinions? Worthwhile? Any experience with it?</div>
> >> <div>=A0</div>
> >> <div>Bob Nienhuis</div>
> >> <div>Neurobiology Research M/S151A3</div>
> >> <div>UCLA / VA Medical Center</div>
> >> <div>North Hills, CA </div>
> >> <div>mailto:
[hidden email]" target=3D"_blank">Bob.Nienhu=
> >>
[hidden email]</div>
> >> </blockquote></div><br>
> >>
> >> --001636e0a6c4223f7c04878b813a--
> >>
> >> ------------------------------
> >>
> >> End of CONFOCALMICROSCOPY Digest - 25 May 2010 to 26 May 2010
> (#2010-16)
> >>
> ************************************************************************>
>