Posted by
Daniel James White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-Poor-man-s-confocal-HiLo-method-tp5112169.html
Hi Christophe,
Begin forwarded message:
>
> Date: Thu, 27 May 2010 11:19:45 +0200
> From: Christophe Leterrier <
[hidden email]>
> Subject: Re: Poor man's confocal
>
> There is at least another cheap and smart method to get optical
> sectioning from a widefield microscope, called "HiLo" microscopy:
>
> Wide-field fluorescence sectioning with hybrid speckle and
> uniform-illumination microscopy.
> Lim D, Chu KK, Mertz J.
> Opt Lett. 2008 Aug 15;33(16):1819-21.
>
> The idea is to take a speckled image and a non speckled one, from
> which high and low frequencies of the images are computed and merged
> to from the final image.
looks very simple.... i wonder if you really need a laser illumination ? Would a metal halide lamp also work?
I dont see why not? Ot does it need to be a coherent beam?
> I don't think there is a commercial version
> of it, but I've seen two other labs using it and it seems quite simple
> to implement.
do you know how what kind of diffuser they use and where to get one?
how did they move the diffuser fast without vibrations?
cheers
Dan
Dr. Daniel James White BSc. (Hons.) PhD
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