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problems with BioRad MRC 600

Posted by Michal Opas on Jun 04, 2010; 9:49pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/magnification-of-digital-microscopes-tp3989284p5141237.html

Well, the List is my last hope.
The problem:
after refurbishing Kr/Ar laser and re-installing it I am not able to get a sharp image on the monitor. My gut feeling is that it probably is not related to laser but a problem with a scan head. So laser outputs fine, staining (as excited by Hg burner) looks spectacular but to get an output pinhole has to be open and gain cranked up. ND filter is on 1. And the image still look blurry.
Any advice as to how to approach the problem shall be greatly appreciated.

Michal

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 Dr. Michal Opas
     Professor
     Department of Laboratory Medicine and Pathobiology
     University of Toronto
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