Re: problems with BioRad MRC 600

Posted by W. Chan on
URL: http://confocal-microscopy-list.275.s1.nabble.com/magnification-of-digital-microscopes-tp3989284p5141291.html

Hi Michael

If it is a new laser tube, there must be something wrong that you have to
use ND position 1 which is 10% transmittance, in addition to a large
pinhole and high gain.  I suspect it's an alignment problem with the laser
coming into the scanhead.  What's the anode current, power measured at the
laser head and at the stage (specimen level)?

If all those are good, then we can try trace along the light path: filter
blocks and other mirrors in the scanhead...

On Fri, 4 Jun 2010, Michal Opas wrote:

> Well, the List is my last hope.
> The problem:
> after refurbishing Kr/Ar laser and re-installing it I am not able to get a
> sharp image on the monitor. My gut feeling is that it probably is not
> related to laser but a problem with a scan head. So laser outputs fine,
> staining (as excited by Hg burner) looks spectacular but to get an output
> pinhole has to be open and gain cranked up. ND filter is on 1. And the image
> still look blurry.
> Any advice as to how to approach the problem shall be greatly appreciated.
>
> Michal

--
Pang (Wai Pang Chan, [hidden email], PAB A087, 206-685-1519)
The Biology Imaging Facility (http://depts.washington.edu/if/)