Re: problems with BioRad MRC 600

Posted by Mark Cannell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/magnification-of-digital-microscopes-tp3989284p5141311.html

Sounds like the alignment is off, not surprising after laser refurbishing.

If you remove the objective with the scan mirrors stationary in the
center of the field, what does the rear aperture illumination look like?
If that is OK, use a mirror as a reflector and examine the alignment on
each folding mirror.

Hope this helps

Mark



Michal Opas wrote:

> Well, the List is my last hope.
> The problem:
> after refurbishing Kr/Ar laser and re-installing it I am not able to
> get a sharp image on the monitor. My gut feeling is that it probably
> is not related to laser but a problem with a scan head. So laser
> outputs fine, staining (as excited by Hg burner) looks spectacular but
> to get an output pinhole has to be open and gain cranked up. ND filter
> is on 1. And the image still look blurry.
> Any advice as to how to approach the problem shall be greatly appreciated.
>
> Michal
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