Embryo time lapse

Posted by Valeria Berno-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Embryo-time-lapse-tp5204753.html

Dear all,

I am running a facility with many different users and I am trying to pull together their needs on imaging in order to improve the equipments.

Right now the biggest demand is about time lapse imaging (long term time lapse (24-48hours with a 15-30min step)) specifically on mouse embryo (E7-E8) with fluorescence (GFP,Venus,dsRed).

So we need very high sensitivity system, weak photo damage, both high magnification (to follow single cells) and possibility to follow movement in the entire embryo.

Too many tasks for a single system?

Which do you think would be the best system to achieve these goals?
a confocal? a multiphoton? the new confocal-stereomicroscope from Nikon AZ-C1? a regular inverted widefield with a good camera?

Which system do you use for these time lapse?

Thanks for all your always helpful input

Valeria

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