Dear Valeria,
A spinning disk does the less
damage to embryos for long term imaging,
Best
Alice Rodriguez Diaz
From: Confocal Microscopy List
[mailto:[hidden email]] On Behalf Of Valeria Berno
Sent: Monday, June 21, 2010 10:19 AM
To: [hidden email]
Subject: Embryo time lapse
Dear all,
I am running a facility with many different users and I am trying to pull
together their needs on imaging in order to improve the equipments.
Right now the biggest demand is about time lapse imaging (long term time lapse
(24-48hours with a 15-30min step)) specifically on mouse embryo (E7-E8) with
fluorescence (GFP,Venus,dsRed).
So we need very high sensitivity system, weak photo damage, both high
magnification (to follow single cells) and possibility to follow movement in
the entire embryo.
Too many tasks for a single system?
Which do you think would be the best system to achieve these goals?
a confocal? a multiphoton? the new confocal-stereomicroscope from Nikon AZ-C1?
a regular inverted widefield with a good camera?
Which system do you use for these time lapse?
Thanks for all your always helpful input
Valeria
--
Valeria Berno,PhD
Microscopy Facility Manager
MRC Centre for Regenerative Medicine
Institute for Stem Cell Research
University of Edinburgh
Roger Land Building
West Mains Road
Edinburgh
EH9 3JQ
Rm 273
Tel Office 0131 6517265