Re: Embryo time lapse

Posted by leoncio vergara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Embryo-time-lapse-tp5204753p5204915.html

I understand Olympus has just released or is about to release a fast autofocus system as well... we want to test it as soon is available

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Armstrong, Brian
Sent: Monday, June 21, 2010 10:44 AM
To: [hidden email]
Subject: Re: Embryo time lapse

Hello, IMHO, I would use a widefield system, an LCD, light engine, or Exfo illumination system, an EMCCD camera, and a quality incubation system that covers the entire scope. You should also consider the laser “focus systems” offered from Zeiss, Nikon, and coming soon Leica (sorry don’t know if Olympus has one yet).

You should be able to accomplish ALL the tasks you outlined.

One thing, if you want to do low mag (5X, 10X) time-lapse imaging you will want a camera with small pixels. The fast EMCCD cameras for high mag have rather large pixels (~16um).

I can talk specs “off-line” if you like.

 

Cheers,

 

Brian D Armstrong PhD

Light Microscopy Core Manager

Beckman Research Institute

City of Hope

Dept of Neuroscience

1450 E Duarte Rd

Duarte, CA 91010

626-256-4673 x62872

http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Valeria Berno
Sent: Monday, June 21, 2010 8:19 AM
To: [hidden email]
Subject: Embryo time lapse

 

Dear all,

I am running a facility with many different users and I am trying to pull together their needs on imaging in order to improve the equipments.

Right now the biggest demand is about time lapse imaging (long term time lapse (24-48hours with a 15-30min step)) specifically on mouse embryo (E7-E8) with fluorescence (GFP,Venus,dsRed).

So we need very high sensitivity system, weak photo damage, both high magnification (to follow single cells) and possibility to follow movement in the entire embryo.

Too many tasks for a single system?

Which do you think would be the best system to achieve these goals?
a confocal? a multiphoton? the new confocal-stereomicroscope from Nikon AZ-C1? a regular inverted widefield with a good camera?

Which system do you use for these time lapse?

Thanks for all your always helpful input

Valeria

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Valeria Berno,PhD

Microscopy Facility Manager

MRC Centre for Regenerative Medicine

Institute for Stem Cell Research

University of Edinburgh

Roger Land Building

West Mains Road

Edinburgh

EH9 3JQ

Rm 273

Tel Office 0131 6517265

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