Posted by
Martin Spitaler on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Embryo-time-lapse-tp5204753p5212831.html
Dear Valeria,
we have done embryo development studies in our facility for 72 hours on a
standard widefield system with standard CCD camera (although a
higher-sensitivity camera would have been desirable, to avoid
phototoxicity). The main problem is definitely the focus, but for fish
embryo development it's not so much the microscope, it's the actual embryo
that starts moving around like crazy after a day, and the thickness of the
sample, so an infrared autofocus will be of limited help (but I would still
go for it for other long time-lapse samples). We just did a few stacks and
picked the interesting ones after completion of time lapse. Just don't
forget to calculate the expected file size before you start, it's going to
be huge! Data storage and transfer is definitely an important consideration.
Good luck,
Martin
======================================
Martin Spitaler, PhD
FILM - Facility for Imaging by Light Microscopy
- Facility Manager -
Sir Alexander Fleming Building, desk 401
Imperial College London / South Kensington
Exhibition Road
London SW7 2AZ
UK
Tel. +44-(0)20-759-42023
E-mail
[hidden email]
Website:
http://imperial.ac.uk/imagingfacility