Re: Preparing figures for publication --PPI vs DPI

Posted by Johannes-P. Koch on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Preparing-figures-for-publication-PPI-vs-DPI-tp5232491p5242437.html

Just to get it right:

Say, I have a CLSM image with 1024x1024px, which was acquired at exc/em
488/520nm using a 63x NA1.4 objective. The zoom factor was adjusted for
Nyquist sampling, let's say such that I have 50nm pixel size.

I have to use these settings, since I want a whole human cell on my
image and I need the resolution as well.

If I consider now the second part of the Nyquist theorem, I would have
to use at least 2 pixels on the print for one pixel in my image - this
would be some 6.8 inches for a 300dpi printer!


This is obviously not feasible anyway! I assume, that only something
like 256x256 px are acceptable for todays image sizes in journals. This
would imply that either I am not "allowed" to use high NA objectives
(because then my field of view is rather limited if I stick to Nyquist)
or I have to crop excessively such that the whole context in a cell is
lost (see settings above). I suppose, you all agree that neither is a
solution. Then, the question remains for me: How can I properly transmit
such an image to a journal. The answer according to all of you is
simply, that it is not possible!

Am I right? This is disastrous.



Johannes

Am 30.06.2010 21:57, schrieb Craig Brideau:

> On Wed, Jun 30, 2010 at 8:29 AM, Robert J. Palmer Jr.
> <[hidden email]>  wrote:
>    
>> 1) does anyone actually read paper journals?  One can lament or applaud the
>> trend towards "electronic only" but it is unstoppable and makes some of this
>> discussion irrelevant.
>>      
> I have to say that 99.9% of the papers I read are PDFs I view on my
> monitor.  Occasionally I will print one out if I want to show it to
> somebody else, but I usually just forward it via email.  That said, it
> is odd that I am viewing an image downsampled in a PDF.  I think it is
> worthwhile to have a downsampled 'thumbnail' or 'preview' image in an
> electronic paper to keep the file size easily emailed/portable.
> However, I think critical images should be hotlinked to the original
> data.  That way I could click on the placeholder/thumbnail image and
> get the raw data to make my own assessment against the author's
> claims.  The tricky part would be to have a static location to store
> the raw data so that the links in the PDF stay good.  Also, the reader
> would have to have an appropriate application to open the data, so
> something free and common, like Fiji/ImageJ would be required.
>
> Craig
>
>    

--
Mag. Johannes-P. KOCH
Department of Biochemistry and Cell Biology
MFPL, University of Vienna
Dr. Bohrgasse 9/5
A-1030 Vienna
Austria

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