Re: Preparing figures for publication --PPI vs DPI

Posted by Jeremy Adler-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Preparing-figures-for-publication-PPI-vs-DPI-tp5232491p5242730.html

It would be simpler if journals would specify the exact width(s) of  
images that they can accommodate in pixels - you can treat dpi as a  
number of pixels.

Making small adjustments to the size of images creates a difference  
between the image you submit and the image as published since the need  
to increase or decrease the number of pixels involves fabricating new  
pixels from the old.

Ideally there should be an integer relationship between the size of  
the original images in pixels as it comes off the microscope and the  
number of pixels in the submitted image. When magnifying an image by  
increasing the number of pixels you should only magnify by an integer  
and specify (Photoshop) that interpolation is by using the nearest  
neighbour. Then one of your original pixels will  still appear to be a  
single pixel when printed.
If necessary pad the final image out with a border but don't give the  
journal an excuse for fiddling with the number of pixels.

Jeremy Adler
Uppsala U
Sweden