Re: quantitative confocal microscopy--photon-counting

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/quantitative-confocal-microscopy-tp5349185p5350052.html

This is a bit of a digression for this thread, but I wonder if anyone
can tell me why photon-counting isn't available on more confocals.  It
seems as if it would be the method of choice for quantitative
experiments--assuming that you're avoiding pile-ups, the counts for a
given wavelength will be directly proportion to the fluorescence.  But
perhaps I'm missing something.

Thanks--

Martin
--
Martin Wessendorf, Ph.D.                   office: [phone]
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