Re: Wavelength to RGB coversion

Posted by Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Wavelength-to-RGB-coversion-tp5381874p5383320.html

There is a real problem with this conversion.  You can take the simplistic option and split the spectrum into three and make those B. G and R but it isn’t actually right – violet will come out as blue.  Photographic films have the same problem.  The way to do it ‘correctly’ is to use the spectral response of the three visual pigments but it’s much harder work and I don’t know of any software which will do this.  Of course it could be done.

 

                                                                                          Guy

 

Optical Imaging Techniques in Cell Biology

by Guy Cox    CRC Press / Taylor & Francis

     http://www.guycox.com/optical.htm

______________________________________________

Associate Professor Guy Cox, MA, DPhil(Oxon)

Australian Centre for Microscopy & Microanalysis,

Madsen Building F09, University of Sydney, NSW 2006

 

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Roshma Azeem
Sent: Saturday, 7 August 2010 7:18 PM
To: [hidden email]
Subject: Re: Wavelength to RGB coversion

 

Hi Esteban,

We too face the same problem with 710 meta and Zeiss has no solution for it. We have to do this one by one. Even with ImageJ, it cannot be done automatically. Though, I tried with ImageTrak in the Mac, I could not do the RGB pseudo coloring simultaneously. I guess, we need to ask Zeiss to develop a plugin for this problem.

Roshma.

On Fri, Aug 6, 2010 at 9:59 PM, Craig Brideau <[hidden email]> wrote:

Our ImageTrak software can handle spectral data and display in RGB.

http://www.ucalgary.ca/styslab/imagetrak

It is Mac only though.

Craig

On Fri, Aug 6, 2010 at 1:21 PM, G. Esteban Fernandez
<[hidden email]> wrote:
> I have 4D (xyz + lambda/spectral) stacks from a Zeiss 710 confocal that I
> need to render in 3D, with the lambda dimension pseudocolored in RGB.
> Zeiss AIM/LSM and ZEN software do not render 3D volumes of lambda-colored
> z-stacks or even export series of lambda-colored planes to TIFF (I'd have to
> do 'em one by one) so I'm going to color each channel according to
> wavelength using ImageJ's Image5D format.  Might there be an ImageJ plugin
> or other software that reads the metadata and does this automatically?
>
> Thanks,
> Esteban

 

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