Colocalization question

Posted by Teng-Leong Chew on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Dyes-for-apoplastic-pH-in-leaf-tissue-tp5505884p5507786.html

Hi all,

Pardon me for opening up a can of worms of colocalization issue.

I wonder if anyone has experience in thresholding a biological
object using a channel (say, green actin in a contractile ring)
and use only that selected ROI to quantify Pearson coeefficient
between green and a red-labeled protein? Bottom line question
is: how much of the red protein co-localizes to green within
the object and not the whole cell?

We have Volocity, MetaMorph and ImageJ.

Thanks in advance for your help.

Regards,
Leong


--
Teng-Leong Chew, Ph.D.
Director, Cell Imaging Facility & Nikon Imaging Center
Director of University Imaging Resources
Feinberg School of Medicine & Office of Research
Northwestern University
303 E. Chicago Avenue
Chicago, IL 60611
[phone]
[phone] (W, F)