Posted by
Armstrong, Louise on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Dyes-for-apoplastic-pH-in-leaf-tissue-tp5505884p5507943.html
*COMMERCIAL RESPONSE*
Dear Leong
From your email I see that you have our Volocity software and so I would
like to direct you to some of our new training videos and tutorials
which I hope will help with your question.
Please visit our website www.cellularimaging.com and look under the
tutorials section.
Alternatively you should be able to click on this link
http://www.cellularimaging.com/Tutorials/On the tutorials page there are two videos about colocalization. The
first by Dr Andrew Barlow deals with the theory of colocalization and
takes you through the interpretation of correlated co-efficients using
simulated and real cell data. The second by Claire Stewart shows you
how to perform colocalization analyses in our latest version of Volocity
5.4, released last month.
If you don't have the latest version of the software then you can
download and try our new free evaluation/demo version and recreate the
steps yourself using some customer/demonstration data. You will find
this here
http://www.cellularimaging.com/products/volocity/demo/
Please feel free to contact me off-list if you would like to discuss
this any further.
Regards
Louise
Louise Armstrong-Denby PhD | Global Product Manager, Bio-discovery
PerkinElmer | For the Better
[hidden email]
www.perkinelmer.com
Please consider the environment before printing this e-mail.
This e-mail message and any attachments are confidential and proprietary
to PerkinElmer, Inc. If you are not the intended recipient of this
message, please inform the sender by replying to this email or sending a
message to the sender and destroy the message and any attachments.
Thank you.
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]]
On Behalf Of Teng-Leong Chew
Sent: 07 September 2010 20:00
To:
[hidden email]
Subject: Colocalization question
Hi all,
Pardon me for opening up a can of worms of colocalization issue.
I wonder if anyone has experience in thresholding a biological
object using a channel (say, green actin in a contractile ring)
and use only that selected ROI to quantify Pearson coeefficient
between green and a red-labeled protein? Bottom line question
is: how much of the red protein co-localizes to green within
the object and not the whole cell?
We have Volocity, MetaMorph and ImageJ.
Thanks in advance for your help.
Regards,
Leong
--
Teng-Leong Chew, Ph.D.
Director, Cell Imaging Facility & Nikon Imaging Center
Director of University Imaging Resources
Feinberg School of Medicine & Office of Research
Northwestern University
303 E. Chicago Avenue
Chicago, IL 60611
(312) 503-2841
(847) 491-7091 (W, F)