Posted by
leoncio vergara on
URL: http://confocal-microscopy-list.275.s1.nabble.com/live-adipose-tissue-tp5552005.html
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We are working on a project involving FLIM imaging of live adipose tissue. We
have experience imaging and keeping alive other tissues but this is the first
time we will attempt to work with fat tissue.
The project involves receiving fresh tissue from biopsies at the begginning of
the day and then keeping them alive during several hrs while we complete the
FLIM imaging sessions on a multiphoton microscope.
The question is how to keep the samples viable outside of the microscope and
on the microscope. We do have the type of equipment commonly used to keep
brain slices alive (imaging chambers, stage incubator, perfusion devices),
however so far I have not found a description about the optimal requirements
to keep adipose tissue viable so we can perform studies that would have
physiological significance.
Also, is there any parameter or dye we can use to monitor viability?
Thanks in advance for your help.
Leoncio Vergara