Re: water refill device for WI objective

Posted by Sylvie Le Guyader-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/water-refill-device-for-WI-objective-tp5566734p5575037.html

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Hi Tom

Thanks for your suggestion. We use the Zeiss oil for water lens routinely
and it works very well as long as one doesn’t try to image a whole multiwell
plate. Oil (normal or for water lenses) is too viscous for large areas. It
collects at places and goes missing at others. We have tried this a lot
without success. The only option we have found to combine higher throughput
and high resolution is to use a water objective. It works really nicely with
glass bottom plates but now we also acquire time lapses, so I guess the only
way to go is to have a water refill system. Hence my query... Does anyone
have any experience with this type of protocols?

Med vänlig hälsning / Best regards
 
Sylvie
 
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Live Cell Imaging Unit
Dept of Biosciences and Nutrition
Karolinska Institutet
Sweden
office: [phone]
LCI room: [phone]
mobile: [phone]

> -----Original Message-----
> From: Donnelly, Tom [mailto:[hidden email]]
> Sent: 24 September 2010 19:11
> To: '[hidden email]'
> Subject: RE: water refill device for WI objective
>
> Sylvie;
>
> I have often thought the oil that Zeiss offers that has the same
refractive index of
> water might be a good solution here.  Once the plate is coated ( probably
do that
> before starting scan) it would seem that little additional oil would be
needed.
>
> I have no experience with this and have no idea if it is compatible with
Nikon

> objectives, but just a thought.
>
> Tom
>
> Tom Donnelly
> Applied Precision, Inc.
> 1040 12th Ave. N.W. Issaquah, WA 98027-8929
> [phone]       [phone] fax
> [hidden email]
> http://www.appliedprecision.com
>
>
>
> -----Original Message-----
> From: Sylvie Le Guyader [mailto:[hidden email]]
> Sent: Friday, September 24, 2010 6:04 AM
> To: [hidden email]
> Subject: water refill device for WI objective
>
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> To join, leave or search the confocal microscopy listserv, go to:
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>
> Dear all
>
>
>
> We have a 60x water objective NA1.2 by Nikon that we would like to use to

screen
> 96 and 384 well plates. Does anyone know of a water refill system that
would fit on
> this objective? Or has anyone tried the Olympus or the Leica water refill
device? Do

> they work well? Would any fit our objective?
>
>
>
> Thanks for your help!
>
>
>
> Med vänlig hälsning / Best regards
>
>
>
> Sylvie
>
>
>
> @@@@@@@@@@@@@@@@@@@@@@@@
>
> Sylvie Le Guyader
>
> Live Cell Imaging Unit
>
> Dept of Biosciences and Nutrition
>
> Karolinska Institutet
>
> Sweden
>
> office: [phone]
>
> mobile: [phone]
>
>
>
>
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