Re: water refill device for WI objective

Posted by Kurt Thorn on
URL: http://confocal-microscopy-list.275.s1.nabble.com/water-refill-device-for-WI-objective-tp5566734p5576129.html

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  I believe Sutter was working on a custom water pump system to refill
water on an objective, but I'm not sure if it came together or not.

I've also wondered if silanizing the bottom of the plate to make it more
hydrophobic would give a more uniform oil distribution.

Kurt

On 9/27/2010 6:49 AM, Sylvie Le Guyader wrote:

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> Hi Tom
>
> Thanks for your suggestion. We use the Zeiss oil for water lens routinely
> and it works very well as long as one doesn’t try to image a whole multiwell
> plate. Oil (normal or for water lenses) is too viscous for large areas. It
> collects at places and goes missing at others. We have tried this a lot
> without success. The only option we have found to combine higher throughput
> and high resolution is to use a water objective. It works really nicely with
> glass bottom plates but now we also acquire time lapses, so I guess the only
> way to go is to have a water refill system. Hence my query... Does anyone
> have any experience with this type of protocols?
>
> Med vänlig hälsning / Best regards
>  
> Sylvie
>  
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader
> Live Cell Imaging Unit
> Dept of Biosciences and Nutrition
> Karolinska Institutet
> Sweden
> office: [phone]
> LCI room: [phone]
> mobile: [phone]
>
>> -----Original Message-----
>> From: Donnelly, Tom [mailto:[hidden email]]
>> Sent: 24 September 2010 19:11
>> To: '[hidden email]'
>> Subject: RE: water refill device for WI objective
>>
>> Sylvie;
>>
>> I have often thought the oil that Zeiss offers that has the same
> refractive index of
>> water might be a good solution here.  Once the plate is coated ( probably
> do that
>> before starting scan) it would seem that little additional oil would be
> needed.
>> I have no experience with this and have no idea if it is compatible with
> Nikon
>> objectives, but just a thought.
>>
>> Tom
>>
>> Tom Donnelly
>> Applied Precision, Inc.
>> 1040 12th Ave. N.W. Issaquah, WA 98027-8929
>> [phone]       [phone] fax
>> [hidden email]
>> http://www.appliedprecision.com
>>
>>
>>
>> -----Original Message-----
>> From: Sylvie Le Guyader [mailto:[hidden email]]
>> Sent: Friday, September 24, 2010 6:04 AM
>> To: [hidden email]
>> Subject: water refill device for WI objective
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> Dear all
>>
>>
>>
>> We have a 60x water objective NA1.2 by Nikon that we would like to use to
> screen
>> 96 and 384 well plates. Does anyone know of a water refill system that
> would fit on
>> this objective? Or has anyone tried the Olympus or the Leica water refill
> device? Do
>> they work well? Would any fit our objective?
>>
>>
>>
>> Thanks for your help!
>>
>>
>>
>> Med vänlig hälsning / Best regards
>>
>>
>>
>> Sylvie
>>
>>
>>
>> @@@@@@@@@@@@@@@@@@@@@@@@
>>
>> Sylvie Le Guyader
>>
>> Live Cell Imaging Unit
>>
>> Dept of Biosciences and Nutrition
>>
>> Karolinska Institutet
>>
>> Sweden
>>
>> office: [phone]
>>
>> mobile: [phone]
>>
>>
>>
>>
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