Posted by
cromey on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Acceptor-Photobleaching-vs-Sensitized-Emission-FRET-results-tp4828946p5632514.html
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Capture an image of a stage micrometer and you will know for sure.
Everything else is just educated guessing.
Doug
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Assistant Scientific Investigator
Dept. of Cell Biology & Anatomy, University of Arizona
1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On
Behalf Of Christophe Leterrier
Sent: Wednesday, October 13, 2010 12:04 PM
To:
[hidden email]
Subject: Re: Pixel size
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If there is no other magnifying/demagnifying optics in your
microscope, the theoretical pixel size is 4.65/40=116.25 nm
Christophe
On Wed, Oct 13, 2010 at 20:43, <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Bonjour à tous,
>
> I have a tiff image, 1392 x 1040 - 4.65 um square pixels acquired with a
> camera interline Sony , 1.4 megapixel, color(7.6mm x 6.2mm array). I use
> a 40 x objective mounted on a table microscope Leica DME.
>
> Can I find the pixel size in hte image?
>
> Thanks ,
>
> Louis
>