Posted by
Andreas Bruckbauer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Acceptor-Photobleaching-vs-Sensitized-Emission-FRET-results-tp4828946p5632770.html
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The problem is that the magnification of microscope objectives can vary a bit from one to another (same make and type). According to one manufacturer this can be +/- 5% for objectives with correction collar and +/- 2% without. Best to measure it but the theoretical pixel size is a good guess if you are sure about the additional magnification optics.
best wishes
Andreas
-----Original Message-----
From: Christophe Leterrier <
[hidden email]>
To:
[hidden email]
Sent: Wed, 13 Oct 2010 20:03
Subject: Re: Pixel size
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If there is no other magnifying/demagnifying optics in your
microscope, the theoretical pixel size is 4.65/40=116.25 nm
Christophe
On Wed, Oct 13, 2010 at 20:43, <
[hidden email]> wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
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> Bonjour à tous,
>
> I have a tiff image, 1392 x 1040 - 4.65 um square pixels acquired with a
> camera interline Sony , 1.4 megapixel, color(7.6mm x 6.2mm array). I use
> a 40 x objective mounted on a table microscope Leica DME.
>
> Can I find the pixel size in hte image?
>
> Thanks ,
>
> Louis
>