Re: perfusion system

Posted by ugez323 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/perfusion-system-tp5674677p5674918.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

You could try an HPLC pump. These quite often meter more than one channel at
flow rates down to 0.1ml/min. Don't know if this would work

Jonathan


Professor J.C. Knowles BSc(hons), PhD, FIMMM, CEng, FRSC, CSci
Professor of Biomaterials Science
Editor, Journal of Biomaterials Applications
Editor, Journal of Tissue Engineering
Head of Division of Biomaterials and Tissue Engineering,
UCL Eastman Dental Institute,
University College London,
256 Gray's Inn Road,
London WC1X 8LD. UK.
Tel +44 (0)207 915 1189
Fax +44 (0)207 915 1227
Mobile 07785 313615
Skype Me. Call name is jknowles_edi
Email [hidden email]
Eastman website; http://www.eastman.ucl.ac.uk
Personal website; http://www.homepages.ucl.ac.uk/~sfhvjck
This email represents the views of the sender alone and must not be
construed as representing the views of the Eastman Dental Institute. It may
contain confidential information and may be protected by law as a legally
privileged document and copyright work. Its content should not be disclosed
and it should not be given or copied to anyone other than the person(s)
named or referenced above. If you have received this email in error, please
contact the sender.



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Hana Uhlirova
Sent: 26 October 2010 14:43
To: [hidden email]
Subject: perfusion system

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear list,

Has anyone experiences with perfusion system for live cell imaging?
Particularly I need to rinse with two liquids of precise concentration. We
have a home-made closed system and have problems with air bubbles and mixing
of those liquids by loss of the concentration accuracy. Any tips how to
figure this out?

Thank you all.

Hana

Hana Uhlirova PhD
Laboratory of Optical Microscopy
Institute of Physical Engineering
Faculty of Mechanical Engineering
Brno University of Technology
Czech Republic