Re: perfusion system

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/perfusion-system-tp5674677p5675740.html

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The pumps are the key.  Syringe pumps or the like are the most reliable, but
provide limited volume.  They are less likely to have air bubbles though.
Don't forget to account for the volume of your delivery tubing when you are
distributing solutions to your sample!
I have had some success with mini gear pumps for delivering about 2ml/min of
solution.  You have to prime the pump first and run it a bit to make sure
any air is out of the system first though.  Peristaltic pumps have similar
issues; run them a bit first to make sure any air is out of the lines.

Craig


On Tue, Oct 26, 2010 at 8:48 AM, <[hidden email]> wrote:

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> You could try an HPLC pump. These quite often meter more than one channel
> at
> flow rates down to 0.1ml/min. Don't know if this would work
>
> Jonathan
>
>
> Professor J.C. Knowles BSc(hons), PhD, FIMMM, CEng, FRSC, CSci
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> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On
> Behalf Of Hana Uhlirova
> Sent: 26 October 2010 14:43
> To: [hidden email]
> Subject: perfusion system
>
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> Dear list,
>
> Has anyone experiences with perfusion system for live cell imaging?
> Particularly I need to rinse with two liquids of precise concentration. We
> have a home-made closed system and have problems with air bubbles and
> mixing
> of those liquids by loss of the concentration accuracy. Any tips how to
> figure this out?
>
> Thank you all.
>
> Hana
>
> Hana Uhlirova PhD
> Laboratory of Optical Microscopy
> Institute of Physical Engineering
> Faculty of Mechanical Engineering
> Brno University of Technology
> Czech Republic
>