Posted by
Tim Feinstein-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/perfusion-system-tp5674677p5676081.html
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Dear Hana,
If your perfusion needs are precise enough then you may want to consider
mixing within the chamber as well as precise control of dispensing (I
agree that two syringe pumps, possibly converging in a Y junction just
before the chamber, may help with dispensing). Mixing within a chamber
can create dramatic and unpredictable gradients at short time scales. For
most applications these do not last long enough to be much of a concern,
but it sounds like you might want to keep it in mind.
One way to make consistent, reproducible perfusion gradients could be to
use a chamber that separates the perfusion input / output from the cells
using a permeable membrane that divides the closed-cell chamber
horizontally. The original idea was to isolate perfused cells from shear
force, but it seems like restricting chaotic mixing to the upper 'chamber'
should also make smoother perfusion gradients. Bioptechs makes one (no
commercial interest).
All the best,
Tim
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>
> Dear list,
>
> Has anyone experiences with perfusion system for live cell imaging?
> Particularly I need to rinse with two liquids of precise concentration. We
> have a home-made closed system and have problems with air bubbles and
> mixing
> of those liquids by loss of the concentration accuracy. Any tips how to
> figure this out?
>
> Thank you all.
>
> Hana
>
> Hana Uhlirova PhD
> Laboratory of Optical Microscopy
> Institute of Physical Engineering
> Faculty of Mechanical Engineering
> Brno University of Technology
> Czech Republic
>
__________________
Timothy Feinstein, PhD
Postdoctoral Associate,
University of Pittsburgh Dept. of Pharmacology and Chemical Biology
BST W1301, 200 Lothrop St.
Pgh, PA 15261