Posted by
DamirSudar on
URL: http://confocal-microscopy-list.275.s1.nabble.com/water-window-x-ray-microscopy-tp5675290p5676228.html
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Researchers at the Advanced Light Source at Lawrence Berkeley National
Laboratory have been working on biological uses of soft X-ray microscopy
and tomography for a while and there are 2 beamlines set up for this at
the ALS. See:
http://ncxt.lbl.gov/?q=technologies for some info about
it and links to further reading. In brief, yes it works on cells and
other biological materials, resolution of about 50-60nm is achievable,
to reduce radiation damage from the X rays samples are fully hydrated
but kept at cryogenic conditions during imaging so no live cells.
- Damir
On 10/26/2010 12:11 PM, Lloyd Donaldson wrote:
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> Having just visited a Synchrotron where this approach was under development (they were still building the beam line and associated imaging equipment) resolution for x-ray tomography may reach sub 100 nm resolution. That is what they are aiming for but I don't think it has been achieved yet. However imaging in water may affect resolution and or contrast. Given the very intense beam it might also kill the cell since you are presumably interested in living tissue. This application is probably more suited to imaging materials.
>
>
> Dr Lloyd Donaldson
>
> Senior Scientist, Project Leader - Microscopy/Wood Identification
> Scion - Next Generation Biomaterials
> Private Bag 3020, Rotorua
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>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Otman Benali
> Sent: Wednesday, 27 October 2010 4:58 a.m.
> To:
[hidden email]
> Subject: water window x-ray microscopy
>
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> Hi ALL,
>
> Water window soft X-ray microscopy is used successfully at Synchrotron
> facilities to image bio samples. Water window is because water is
> transparent to soft X-rays and only carbon constituents of the cell are
> imaged. Could you please let me know how this techniques compares to other
> microscopy techniques for bio applications.
>
> Many thanks,
> Otman BENALI, PhD
>
> NANO-UV SAS, Paris/FRANCE
>
http://www.nanouv.com>
http://www.mcxi.eu>
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--
Damir Sudar - Staff Scientist and Deputy for Technology
Lawrence Berkeley Laboratory / Life Sciences Division
One Cyclotron Road, MS 977R225A, Berkeley, CA 94720, USA
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WWW:
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