Re: water window x-ray microscopy

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URL: http://confocal-microscopy-list.275.s1.nabble.com/water-window-x-ray-microscopy-tp5675290p5677750.html

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Dear Ian,

Many thanks. The X-radia system is based on hard X-rays. Water window
refers to microscopy at wavelength between 2 and 4nm where water is
relatively transparent and only Carbon and Nitrogen constituents of the
cell are imaged. The technology exists using Synchrotron light but there
are a few tabeltop system being developed based on discharge and laser
produced plasma light sources.

Regards,

Otman BENALI, PhD

NANO-UV SAS

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On 26/10/2010 23:07, Ian Hallett wrote:

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> Lloyd
>
> In terms of X-ray micro tomography resolution non synchrotron systems now claim resolutions down to 50 nm (for example the Ultra from Xradia).  I'm not sure how well these work in practice for wet biological material.
>
> Ian
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> Ian Hallett
> Senior Scientist
> Team Leader: Microscopy and Cell Walls
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> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Lloyd Donaldson
> Sent: Wednesday, 27 October 2010 8:11 a.m.
> To: [hidden email]
> Subject: Re: water window x-ray microscopy
>
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> Having just visited a Synchrotron where this approach was under development (they were still building the beam line and associated imaging equipment) resolution for x-ray tomography may reach sub 100 nm resolution. That is what they are aiming for but I don't think it has been achieved yet. However imaging in water may affect resolution and or contrast. Given the very intense beam it might also kill the cell since you are presumably interested in living tissue. This application is probably more suited to imaging materials.
>
>
> Dr Lloyd Donaldson
>
> Senior Scientist, Project Leader - Microscopy/Wood Identification
> Scion - Next Generation Biomaterials
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> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Otman Benali
> Sent: Wednesday, 27 October 2010 4:58 a.m.
> To: [hidden email]
> Subject: water window x-ray microscopy
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> Hi ALL,
>
> Water window soft X-ray microscopy is used successfully at Synchrotron
> facilities to image bio samples. Water window is because water is
> transparent to soft X-rays and only carbon constituents of the cell are
> imaged. Could you please let me know how this techniques compares to other
> microscopy techniques for bio applications.
>
> Many thanks,
> Otman BENALI, PhD
>
> NANO-UV SAS, Paris/FRANCE
> http://www.nanouv.com
> http://www.mcxi.eu
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