Posted by
Teng-Leong Chew on
URL: http://confocal-microscopy-list.275.s1.nabble.com/perfusion-system-tp5674677p5679227.html
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Hi Hana,
Mixing of liquid within the chamber is rarely accurate,
especially if you need the drug effect to kick in instantly
(since I don't know what you are working on, it is hard
to give precise recommendation).
Using peristaltic pump may also create "breathing" effect
as you are imaging. So your set-up should be in such a
way that the chamber is never connected to the pump
directly, but through a liquid reservoir that can help
dampen the breathing effect. Syringe pump works better
in this case.
There are a lot of microprocessor controlled, microfluidic
systems that you can buy if you have the cash. But I am
a happy Bioptechs customer (no $$ interest or connection).
--
Teng-Leong Chew, Ph.D.
Director, Cell Imaging Facility & Nikon Imaging Center
Director of University Imaging Resources
Feinberg School of Medicine & Office of Research
Northwestern University
303 E. Chicago Avenue
Chicago, IL 60611
(312) 503-2841
(847) 491-7091 (W, F)
> Dear list,
>
> Has anyone experiences with perfusion system for live cell imaging?
> Particularly I need to rinse with two liquids of precise concentration.
> We
> have a home-made closed system and have problems with air bubbles and
> mixing
> of those liquids by loss of the concentration accuracy. Any tips how to
> figure this out?
>
> Thank you all.
>
> Hana
>
> Hana Uhlirova PhD
> Laboratory of Optical Microscopy
> Institute of Physical Engineering
> Faculty of Mechanical Engineering
> Brno University of Technology
> Czech Republic