Re: perfusion system

Posted by Jeremy Adler-3 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/perfusion-system-tp5674677p5681546.html

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The cyclical pressure changes produced by roller pumps will cause  
movement of the chamber and a change in focus.
This can be eliminated by (a) using a air filled side arm which damps  
the pulsations, as a been mentioned previously, and (b) by making the  
outlet port of the perfusion chamber much wider than the inlet port -  
something rarely seen in commercial perfusions.

Gravity provides pulseless driving force for perfusions and is quite cheap.

Jeremy Adler
Genetics & Pathology
Rudbeckslaboratoriet
Daghammersköljdsväg 20
751 85 Uppsala
Sweden

0046 (0)18 471 4607