Posted by
mmodel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Basic-live-cell-imaging-question-tp5687698p5687906.html
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If you don't need fluorescence you can observe live cells in Petri dishes using phase of Hoffman contrast.
Mike
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From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Mika Hukkanen
Sent: Friday, October 29, 2010 4:08 PM
To:
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Subject: Re: Basic live cell imaging question...
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Hi Colin, we do it almost on a daily basis and with various settings; simple
and relatively inexpensive - just get a long distance dip type objective
from Olympus. We have got 2 sets from Leica; 1 set for mammalian cells and 1
for infectious samples (viruses, bacteria etc) - all for live cell studies.
Numerical aperture is not high, but sufficient enough for single cell
studies. High NA dip objectives are very, very bulky and expensive, but
these ones with ceramic head (meant for physiological imaging) are within
very manageable budget. Olympus manufactures really excellent objectives,
just contact the local rep - or us for further info on the use of these 'dip
type' objectives etc.
Mika
-----Original Message-----
From: Confocal Microscopy List [mailto:
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Behalf Of Dolphin, Colin
Sent: 29. lokakuuta 2010 22:01
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Subject: Basic live cell imaging question...
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We would like to do live cell imaging - mammalian cell lines - but only have
direct access to an upright Olympus BX61. We don't really need complicated
perfusion chambers, etc just something simple. We're real neophytes so all
suggestions gratefully received.
Colin