Re: Basic live cell imaging question...

Posted by Gert van Cappellen-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Basic-live-cell-imaging-question-tp5687698p5703062.html

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  Culture your cells on a round coverslip. Take an object glas glue a
square piece of non-toxic rubber with a round hole on it. Fill this with
CO2 satured medium somewaht more as the volume of the hole. Put your
coverslip on it, with the cells to the medium off course. Press it
gently down and the glass will seal itself to the rubber ring. Now your
cells will survive for a couple of hours, so you can do the first
imaging. For real experiments you have to find a way to heat the object
glass to 37C.

Good luck, Gert

Op 29-10-2010 21:00, Dolphin, Colin schreef:
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> We would like to do live cell imaging - mammalian cell lines - but only have direct access to an upright Olympus BX61. We don't really need complicated perfusion chambers, etc just something simple. We're real neophytes so all suggestions gratefully received.
>
> Colin
>