Posted by
Axel Kurt Preuss on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Basic-live-cell-imaging-question-tp5687698p5703826.html
You need a water immersion object or have to build one
Cheers
Axel
—————
Axel K Preuss, PhD,
Central Imaging, IMCB, A*Star, 61 Biopolis Dr, 6-19B, Singapore 138673, sent from 9271.5622
On Nov 4, 2010, at 4:06 AM, Gert van Cappellen <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Culture your cells on a round coverslip. Take an object glas glue a
> square piece of non-toxic rubber with a round hole on it. Fill this with
> CO2 satured medium somewaht more as the volume of the hole. Put your
> coverslip on it, with the cells to the medium off course. Press it
> gently down and the glass will seal itself to the rubber ring. Now your
> cells will survive for a couple of hours, so you can do the first
> imaging. For real experiments you have to find a way to heat the object
> glass to 37C.
>
> Good luck, Gert
>
> Op 29-10-2010 21:00, Dolphin, Colin schreef:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>> *****
>>
>> We would like to do live cell imaging - mammalian cell lines - but only have direct access to an upright Olympus BX61. We don't really need complicated perfusion chambers, etc just something simple. We're real neophytes so all suggestions gratefully received.
>>
>> Colin
>>
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