http://confocal-microscopy-list.275.s1.nabble.com/Basic-live-cell-imaging-question-tp5687698p5706861.html
normally this gives enough information for single cells. However a water
> You need a water immersion object or have to build one
>
>
> Cheers
>
> Axel
> —————
> Axel K Preuss, PhD,
> Central Imaging, IMCB, A*Star, 61 Biopolis Dr, 6-19B, Singapore 138673, sent from 9271.5622
>
>
> On Nov 4, 2010, at 4:06 AM, Gert van Cappellen<
[hidden email]> wrote:
>
>> *****
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>>
>> Culture your cells on a round coverslip. Take an object glas glue a
>> square piece of non-toxic rubber with a round hole on it. Fill this with
>> CO2 satured medium somewaht more as the volume of the hole. Put your
>> coverslip on it, with the cells to the medium off course. Press it
>> gently down and the glass will seal itself to the rubber ring. Now your
>> cells will survive for a couple of hours, so you can do the first
>> imaging. For real experiments you have to find a way to heat the object
>> glass to 37C.
>>
>> Good luck, Gert
>>
>> Op 29-10-2010 21:00, Dolphin, Colin schreef:
>>> *****
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>>> We would like to do live cell imaging - mammalian cell lines - but only have direct access to an upright Olympus BX61. We don't really need complicated perfusion chambers, etc just something simple. We're real neophytes so all suggestions gratefully received.
>>>
>>> Colin
>>>
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