Posted by
Straatman, Kees (Dr.) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Basic-live-cell-imaging-question-tp5687698p5708177.html
I always wonder if a water immersion lens is better than an oil immersion lens for live cell imaging. Both have the wrong RI for cells. Water too low, oil too high. However, the NA of an oil lens is higher than from a water lens, so the oil objective should be more light efficient and your resolution should be a little better. Or am I wrong....?
kees
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Gert van Cappellen
Sent: 04 November 2010 20:38
To:
[hidden email]
Subject: Re: Basic live cell imaging question...
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I'm quite sure the cells will also survive an oil immersion lens and
normally this gives enough information for single cells. However a water
immersion lens is better but certainly not necessary.
Best regards,
Gert
Op 4-11-2010 2:03, Axel Kurt Preuss schreef:
> You need a water immersion object or have to build one
>
>
> Cheers
>
> Axel
> —————
> Axel K Preuss, PhD,
> Central Imaging, IMCB, A*Star, 61 Biopolis Dr, 6-19B, Singapore 138673, sent from 9271.5622
>
>
> On Nov 4, 2010, at 4:06 AM, Gert van Cappellen<
[hidden email]> wrote:
>
>> *****
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>>
>> Culture your cells on a round coverslip. Take an object glas glue a
>> square piece of non-toxic rubber with a round hole on it. Fill this with
>> CO2 satured medium somewaht more as the volume of the hole. Put your
>> coverslip on it, with the cells to the medium off course. Press it
>> gently down and the glass will seal itself to the rubber ring. Now your
>> cells will survive for a couple of hours, so you can do the first
>> imaging. For real experiments you have to find a way to heat the object
>> glass to 37C.
>>
>> Good luck, Gert
>>
>> Op 29-10-2010 21:00, Dolphin, Colin schreef:
>>> *****
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>>> We would like to do live cell imaging - mammalian cell lines - but only have direct access to an upright Olympus BX61. We don't really need complicated perfusion chambers, etc just something simple. We're real neophytes so all suggestions gratefully received.
>>>
>>> Colin
>>>
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