http://confocal-microscopy-list.275.s1.nabble.com/Basic-live-cell-imaging-question-tp5687698p5708384.html
On Fri, Nov 5, 2010 at 09:51, Straatman, Kees R. (Dr.) <
> I always wonder if a water immersion lens is better than an oil immersion
> lens for live cell imaging. Both have the wrong RI for cells. Water too low,
> oil too high. However, the NA of an oil lens is higher than from a water
> lens, so the oil objective should be more light efficient and your
> resolution should be a little better. Or am I wrong....?
>
> kees
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
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> On Behalf Of Gert van Cappellen
> Sent: 04 November 2010 20:38
> To:
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> Subject: Re: Basic live cell imaging question...
>
> *****
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> I'm quite sure the cells will also survive an oil immersion lens and
> normally this gives enough information for single cells. However a water
> immersion lens is better but certainly not necessary.
>
> Best regards,
> Gert
>
> Op 4-11-2010 2:03, Axel Kurt Preuss schreef:
> > You need a water immersion object or have to build one
> >
> >
> > Cheers
> >
> > Axel
> > —————
> > Axel K Preuss, PhD,
> > Central Imaging, IMCB, A*Star, 61 Biopolis Dr, 6-19B, Singapore 138673,
> sent from 9271.5622
> >
> >
> > On Nov 4, 2010, at 4:06 AM, Gert van Cappellen<
>
[hidden email]> wrote:
> >
> >> *****
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> >>
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> >>
> >> Culture your cells on a round coverslip. Take an object glas glue a
> >> square piece of non-toxic rubber with a round hole on it. Fill this with
> >> CO2 satured medium somewaht more as the volume of the hole. Put your
> >> coverslip on it, with the cells to the medium off course. Press it
> >> gently down and the glass will seal itself to the rubber ring. Now your
> >> cells will survive for a couple of hours, so you can do the first
> >> imaging. For real experiments you have to find a way to heat the object
> >> glass to 37C.
> >>
> >> Good luck, Gert
> >>
> >> Op 29-10-2010 21:00, Dolphin, Colin schreef:
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>>
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> >>>
> >>> We would like to do live cell imaging - mammalian cell lines - but only
> have direct access to an upright Olympus BX61. We don't really need
> complicated perfusion chambers, etc just something simple. We're real
> neophytes so all suggestions gratefully received.
> >>>
> >>> Colin
> >>>
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