TIRF depth calibration

Posted by Graham Wright on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-depth-calibration-tp5769934.html

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Hello,

Does anyone have a routine way of calibrating the effective penetration
depth of TIRF imaging?

I've found this paper:

Gell, et al. 2009 TIRF microscopy evanescent field calibration using tilted
fluorescent microtubules. Journal of Microscopy 234: 38-46
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.2009.03147.x/references

but wondered if other people have different ways?

Thanks,
Graham

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Graham Wright

Institute of Medical Biology
8A Biomedical Grove, #06-06 Immunos, Singapore 138648

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