Re: TIRF depth calibration

Posted by Sebastian Rhode on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-depth-calibration-tp5769934p5770091.html

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Hi Graham,

one method which definitly works (I tried it out by myself) is the use of an
combined TIRF-AFM setup. You just have to couple fluorescent beads to the
tip of your AFM an record pictures while approaching/or moving away the
coverslip surface. Unfortunately an AFM is really expensive.

So I found some other methods, which might work as well --> see
TIRF_Introduction.pdf, which I send to you directly (LIST server does not
accepted this pdf-file).

One methodes uses an objective piezo-drive and a pencil and the second one
stained beads or a stained solution with intransparent beads.

I case of questions, feel free to contact me directly.

Cheers,
Sebastian


Dr. Sebastian Rhode
Project Manager
Research & Development

TILL Photonics GmbH
Lochhamer Schlag 21
D- 82166 Gräfelfing, Germany
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www.till-photonics.com