Re: Far red FP for 2P imaging

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/timeseries-large-images-and-focus-tp5747682p5771519.html

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I seem to recall some preliminary work with Chromium:Forsterite lasers with
longer dyes.  Cr:Forsterite picks up at about 1200-1300nm so apparently it
is a good wavelength range to stimulate the really red dyes ~700-800nm alexa
dyes and the like.  You'd need red extended detectors to actually pick up
the signal from the dye though.

Craig

On Wed, Nov 24, 2010 at 8:45 AM, Deepak nair <[hidden email]>wrote:

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> Hi,
>
> I do not know much about the 2photon behaviour but what about the far red
> proteins IFP from Tsien lab and Crimson from the lab of Ben Glick
>
> Deepak
>
> On Wed, Nov 24, 2010 at 4:28 PM, Koo, Lily (NIH/NIAID) [E] <
> [hidden email]> wrote:
>
> > *****
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> > Dear List,
> >
> > Is there a far red FP you'd recommend for 2-photon imaging in addition to
> > green/red pairs such as GFP/dsRED?
> >
> > Thanks,
> >
> > Lily
> >
>
>
>
> --
>  Deepak Nair, PhD
> "Physiologie Cellulaire de la Synapse"
> UMR 5091 CNRS
> Université Bordeaux 2
> Institut François Magendie
> 33077 Bordeaux Cedex
> Tél. : 05 57 57 40 81
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