Posted by
Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/timeseries-large-images-and-focus-tp5747682p5772685.html
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There is a problem with doing 2P imaging within the tunable range of
your excitation laser, since the microscope will have dichroics and
blocking filters to cut out that part of the spectrum. You'd need to
change some filters - and, for safety's sake, probably put in some
interlock to prevent your laser being tuned into the danger range.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]]
On Behalf Of Koo, Lily (NIH/NIAID) [E]
Sent: Thursday, 25 November 2010 2:28 AM
To:
[hidden email]
Subject: Far red FP for 2P imaging
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Dear List,
Is there a far red FP you'd recommend for 2-photon imaging in addition
to green/red pairs such as GFP/dsRED?
Thanks,
Lily