http://confocal-microscopy-list.275.s1.nabble.com/TIRF-depth-calibration-tp5769934p5775891.html
> *****
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>
> Another publication related to this topic is this one:
>
>
http://spiedl.aip.org/getabs/servlet/GetabsServlet?prog=normal&id=JBOPFO000011000001014006000001&idtype=cvips&gifs=yes&ref=no> Mattheyses, A.L. and Axelrod, D.
> Direct measurement of the evanescent field profile produced by
> objective-based total internal reflection fluorescence
> J. Biomed. Opt., Vol. 11, 014006 (2006)
>
> Did not try it (yet), however.
>
> Best wishes
>
> Lauran Oomen
> --------------------------------------------------------------
> Lauran Oomen
> Manager Digital Microscopy Facility (C.2.023)
> NKI-AVL
> Plesmanlaan 121
> PO Box 90203
> 1006 BE Amsterdam
> The Netherlands
>
> phone +31 205126080
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]]
> On Behalf Of John Oreopoulos
> Sent: woensdag 24 november 2010 17:21
> To:
[hidden email]
> Subject: Re: TIRF depth calibration
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> And for the more adventurous types out there, you can measure your
> penetration depth using fluorescence correlation spectroscopy (FCS) if you
> have the right hardware:
>
> Harlepp, S., et al., Subnanometric measurements of evanescent wave
> penetration depth using total internal reflection microscopy combined with
> fluorescent correlation spectroscopy. Applied Physics Letters, 2004. 85(17):
> p. 3917-3919.
>
> John Oreopoulos
> Research Assistant
> Spectral Applied Research
> 9078 Leslie Street, Unit 11
> Richmond Hill
> Ontario, Canada
>
>
> On 2010-11-24, at 10:37 AM, Christophe Leterrier wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > *****
> >
> > There is also a simple method in Fiolka et al. Miocroscoc. res Tech 2007
> :
> >
http://www.neuro.nano-optics.ethz.ch/publications/fiolka.pdf> >
> > <
http://www.neuro.nano-optics.ethz.ch/publications/fiolka.pdf>involving
> an
> > inclined coverslip with attached fluorescent beads. I didn't try it
> myself
> > so I can't tell if it works, but seems simpler than AFM or microtubules
> > method.
> >
> > Christophe
> >
> > On Wed, Nov 24, 2010 at 16:32, John Oreopoulos
> > <
[hidden email]>wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >> *****
> >>
> >> Sebastian, I remember reading a publication from about ten years ago
> that
> >> talked about mounting a pencil in a piezoelectric micro-manipulator and
> >> sticking a fluorescent bead on the end of the pencil tip. This was very
> >> similar to the AFM method. Is it the same thing?
> >>
> >> Graham, there is a fairly detailed discussion on this topic from a few
> >> years ago on the archive that talks about a few other ways to measure
> the
> >> actual TIRF penetration depth (as opposed to calculating it based on an
> >> assumed refractive index and crudely measured incident angle):
> >>
> >>
> >>
>
http://lists.umn.edu/cgi-bin/wa?A3=ind0702&L=CONFOCALMICROSCOPY&E=quoted-printable&P=827770&B=--Apple-Mail-181-588547976&T=text%2Fhtml;%20charset=ISO-8859-1> >>
> >> I've tried the AFM method as well - it works, but my main complaint with
> >> this and some of the other protocols is that they require complicated
> and
> >> expensive equipment, and can be difficult to get right. The method
> involving
> >> fluorescent microtubles by Jorg Enderlein's group is a fairly new one
> that
> >> is elegantly simple, but again requires you to have access to some
> fairly
> >> special reagents that might not be found in every lab. A few months ago
> I
> >> came across yet another older method that had evaded my previous
> searches on
> >> the topic. This one is similar to the others that involve imaging
> >> fluorescent microbeads, but I like this because all it requires is a
> >> microscope with a motorized drive on the z-axis:
> >>
> >> Steyer, J.A. and W. Almers, Tracking single secretory granules in live
> >> chromaffin cells by evanescent-field fluorescence microscopy.
> Biophysical
> >> Journal, 1999. 76(4): p. 2262-2271.
> >>
> >> John Oreopoulos
> >> Research Assistant
> >> Spectral Applied Research
> >> 9078 Leslie Street, Unit 11
> >> Richmond Hill
> >> Ontario, Canada
> >>
> >>
> >> On 2010-11-24, at 6:54 AM, Sebastian Rhode wrote:
> >>
> >>> *****
> >>> To join, leave or search the confocal microscopy listserv, go to:
> >>>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> >>> *****
> >>>
> >>> Hi Graham,
> >>>
> >>> one method which definitly works (I tried it out by myself) is the use
> of
> >> an
> >>> combined TIRF-AFM setup. You just have to couple fluorescent beads to
> the
> >>> tip of your AFM an record pictures while approaching/or moving away the
> >>> coverslip surface. Unfortunately an AFM is really expensive.
> >>>
> >>> So I found some other methods, which might work as well --> see
> >>> TIRF_Introduction.pdf, which I send to you directly (LIST server does
> not
> >>> accepted this pdf-file).
> >>>
> >>> One methodes uses an objective piezo-drive and a pencil and the second
> >> one
> >>> stained beads or a stained solution with intransparent beads.
> >>>
> >>> I case of questions, feel free to contact me directly.
> >>>
> >>> Cheers,
> >>> Sebastian
> >>>
> >>>
> >>> Dr. Sebastian Rhode
> >>> Project Manager
> >>> Research & Development
> >>>
> >>> TILL Photonics GmbH
> >>> Lochhamer Schlag 21
> >>> D- 82166 Gräfelfing, Germany
> >>> Phone +49 (0)89 895 662-120
> >>> Fax +49 (0)89 895 662-101
> >>> www.till-photonics.com
> >>
>