Posted by
Gordon Scott on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Guidance-wanted-on-illumination-stability-tp5784748p5791211.html
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Hi Jeremy,
> [mailto:
[hidden email]] On Behalf Of Jeremy Adler
> It might also be useful to have pulsatile LEDs operating in
> the nanosecond range - there was a Stefan Hell paper that
> showed very significant reductions in photobleaching when the
> gap between pulses was longer than the fluorescent lifetime.
> Is this possible with LEDs ?
Pulses of multiple nanoseconds duration should be feasible enough,
though not with the architecture of our present products, which are
speed constrained due to the smoothing/linearisation we use. Our present
units run to around 10kHz and we know we can make them run to around
300kHz with some modest changes that will likely roll out in the
standard product in the moderately near future.
To do nanosecond pulses really needs a product designed specifically for
that kind of speed and the smoothing/linearisation becomes irrelevant.
At those speeds, one changes from linear control to packets of energy.
I'd like to better understand what the constraints, limits and future
potentials are in the technique. There are some physics constraints at
present on the intensity levels that are feasible with LEDs, which might
leave the technique in the realm of lasers for a while.
Thanks for the feedback.
Kind regards,
Gordon.
--
Gordon Scott Design Engineering
Custom Interconnect Ltd.
http://www.cil-uk.co.uk CoolLED
http://www.coolled.com CoolLED is a division of Custom Interconnect Ltd.
Phone +44-1264-321321
CIL House, Charlton Road, Andover SP10 3JL, UK
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