Posted by
Guy Cox-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Guidance-wanted-on-illumination-stability-tp5784748p5798334.html
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The funny thing is that Bangs list, in their spectra, "Far out red"
which seems to be exactly the colour you want - but they don't seem to
offer many (any?) actual beads with this dye.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
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-----Original Message-----
From: Confocal Microscopy List [mailto:
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On Behalf Of Clements, Ian
Sent: Friday, 3 December 2010 10:27 AM
To:
[hidden email]
Subject: Re: 100 nm PSF kit
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We do use these but for certain applications we need single color beads.
Also the TetraSpecks are not quite as bright but it may be all we can do
right now.
Ian Clements
Product Manager - DeltaVision OMX Systems
-----Original Message-----
From: Andreas Bruckbauer [mailto:
[hidden email]]
Sent: Thursday, December 02, 2010 2:10 PM
To:
[hidden email]
Subject: Re: 100 nm PSF kit
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What about 0.1 micrometer TetraSpeck beads from Molecular Probes? They
are about 110 +/- 6 nm in size (might depend on the batch) and light up
in all channels inclusive the far red. However they seem to loose their
fluorescence over time and are useless after a month or so.
best wishes
Andreas
-----Original Message-----
From: Clements, Ian <
[hidden email]>
To:
[hidden email]
Sent: Thu, 2 Dec 2010 21:56
Subject: Re: 100 nm PSF kit
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Apologies to group for mis-directed message but happy to hear from
anyone who
has a source of these.
Ian
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