Posted by
Emmanuel Gustin on
URL: http://confocal-microscopy-list.275.s1.nabble.com/TIRF-depth-calibration-tp5769934p5808557.html
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Hi Louis,
We have such a system, on a PerkinElmer Opera. We find that it works reliably if you take basic, sensible precautions: Make sure all connectors are tight, sonicate the immersion water to degas it and reduce the formation of bubbles in the fluid path, clean the collection channels regularly to prevent them getting clogged by dust, flush the system a few minutes before every run. It is suitable for long runs.
As for it being 'realistic', it all depends on what your application needs. There is no single best option for HCS experiments... Almost all HCS labs have several different instruments to choose from, dependent on the application, and having a low-resolution and a high-resolution system side-by-side is fairly typical.
High-NA water immersion objectives are a good tool if you are looking at small cells, want to see sub-cellular structural detail, have weak signals, or require good Z-sectioning performance on a confocal system. But they have disadvantages as well, such as short working distances, a requirement to have high-quality plate bottoms, and a relatively small field of view as they are all (relatively) high magnification.
Best Regards,
Emmanuel
--
Emmanuel Gustin, Tel. (+32) 14 64 1586, e-mail:
[hidden email] ! telephone number changed !
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of
[hidden email]
Sent: maandag 6 december 2010 16:26
To:
[hidden email]
Subject: HCS microscopy-immersion (water/oil) dispenser
Bojour à tous,
I would like some information/feellings about immersion (water/oil)
dispenser for the use high resolution objective in the context of High
content screening microscopy.
I would like to know if those device works well for imaging a lot of
plates? Is it reliable?
Would it be more realistic to use lower NA objective (without immersion)
for HCS microscopy?
Thanks for your help,
Louis