DsRed vs 2P

Posted by Staffan Nyström on
URL: http://confocal-microscopy-list.275.s1.nabble.com/DsRed-vs-2P-tp5830859.html

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Dear list members,

Has anyone any experience with visualizing DsRed using Ti:Saph (2 photon)?
I have problems getting any decent fluorescence signal through between 700 and 1000nm excitation and there is significant bleedthrough
with EGFP. In the end I gave up since the "regular" 1 photon excitation way gave a much better S/N ratio with minimal bleed through.

Any tips? We have an upright Zeiss LSM system and mostly use 40X 1.0 NA water dipping objectives.

Best

Staffan Nystrom

PhD student

MBB / Oncology & Pathology

Karolinska Institutet
Stockholm, Sweden