Re: Mowiol and 3D imaging

Posted by ChrisWilms on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Mowiol-and-3D-imaging-tp5845120p5857106.html

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Dear all,

thanks for your responses on this. Sounds as if Mowiol has the same  
problems as most other low RI and/or curing mountants.

As far as I gathered, the RI of Mowiol is too low for good axial  
measurements even when solidified. I'm still trying to chase down a  
mounting medium with a RI similar to immersion oil that doesn't quench  
fluorescence excessively. I've tried TDE and actual immersion oil --  
both gave pretty dim images in contrast to other mounting media  
(Vectashield, Prolong). I'm now trying several of Citifluor's, hoping  
to find a good mix.

Again, thanks for the responses!

Best, Christian