Re: Stage micrometers and diatoms

Posted by Steffen Dietzel on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Stage-micrometers-and-diatoms-tp589976p589978.html

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At 14:37 10.08.2007, you wrote:

>  Is there a stage micrometer that works in both
> transmission and reflection mode- for measuring true magnification?

Hi Shalin,

I used a USAF 1951 target for exactly that
purpose. It has a mirror surface with a clear
pattern on it. It appears there are several
vendors for those. I got it from Edmund
http://www.edmundoptics.com/

I believe the one that I used currently costs 111
Euros in Germany (cat. no. Y38-256). I switched
work places and just reordered it, so I can't
check the cat number on the slide itself.

One note of caution: I had some trouble with a
version of the slide that contains fluorescent
material. The focal plain is so far away from the
surface, that the working distance of my oil
immersion objectives was too short to reach it.
So you're better off with the one with the
surface mirror and use that in reflection or
transmission mode, as you plan anyway.

Steffen

--
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum (WBZ)
Head of light microscopy

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